Projects & Grants
| Evaluation of the Effectiveness of Disinfectants Against Multidrug-Resistant Gram-Negative Bacteria | |
|---|---|
| Project Id | SGS06/LF/2026 |
| Main solver | Mgr. Karolína GODI |
| Period | 1/2026 - 12/2026 |
| Provider | Specifický VŠ výzkum |
| State | solved |
| Anotation | Healthcare-associated infections (HAIs) represent a major challenge, particularly among critically ill patients. The proper selection and use of disinfectants are key components in preventing their spread. However, the effectiveness of disinfectants can be influenced by several factors, including the composition of active substances, the type of microorganism, and the presence of organic load in the environment. Studies indicate that disinfectants based on quaternary ammonium compounds (QACs) and chlorine-based agents (CBAs) may show reduced efficacy under certain conditions, especially in the presence of biofilm or protein load, which can affect bactericidal activity against multidrug-resistant (MDR) bacterial strains. The project focuses on evaluating the effectiveness of commonly used disinfectants representing the main groups of active compounds ? QACs, CBAs, and alcohol-based disinfectants used for hand hygiene. Both hand and surface disinfectants applied in healthcare settings will be tested. In total, five disinfectant products with different chemical compositions and two modes of application will be evaluated to compare the efficacy of various active substance groups. Efficacy assessment will be performed using the suspension micromethod, determining the minimum bactericidal concentration (MBC) and MBC with protein load (MBC-B). This method has been previously validated in a master?s thesis (Godi, 2022), which demonstrated differences in efficacy among products and the significant impact of organic load. The results of the proposed project will enable objective comparison of disinfectant efficacy, evaluation of the effect of protein interference, and formulation of recommendations for optimizing disinfection protocols in healthcare facilities, ultimately contributing to improved patient safety. |
| Total Costs | 262 900 CZK |
